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Product Description
Wayne293® Expression Medium is a protein-free medium specifically developed for the supporting the growth and transfection. It is compatible and applicable to cell lines HEK293, HEK293T, HEK293A, and HEK293FT under suspension culture conditions. Wayne293™ Expression Medium is a complete, ready-to-use and animal-origin free medium.
Product Procedure
Product Components
| L-glutamine | 4mM |
| Glucose | 6.0 g/L |
| Hypoxanthine & Thymidine | No |
| Phenol red | No |
| Sodium bicarbonate | Yes |
| Hydrolysate | Non-animal origin Pepton |
High-density value-added:
The growth of different HEK293 cells in Wayne293™. Batch, 125mL shake flask, working volume 30mL, ф25mm, 125rpm, humidity 75%, 5% CO2, 37°C for 7 days.
Continuous passage growth and transient expression of HEK293 F cells in Wayne293™.
125mL shake flask, working volume 30mL, ф25mm, 125rpm, humidity 75%, 5%CO237℃ culture; passage the next day, 20 passages in total, cryopreserved every 5 generations;
unified recovery after passage, and transfection according to the standard method of instruction An IgG plasmid to detect the amount of transient expression.
High-yield expression:
Growth and expression of HEK293 F cells after transfection in Wayne293™ and other commercial media.
Control: Medium F is supplier G's HEK293 transient medium; 125mL shake flask, working volume 30mL,ф25mm, 125rpm, humidity 75%, 5% CO2, 37℃ culture;
according to the standard method of the instruction transfect three expressions respectively Different IgG plasmids were cultured for 7 days.
24 hours after transfection of HEK293F cells in Wayne293 TM and other commercial media
Certificate of Analysis (COA)
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Protocol & Documents
